Introduction
The Human Apoptosis Antibody Array (Catalog # ARY009) is a rapid, sensitive, and economical tool to simultaneously detect the relative expression of 35 apoptosis-related proteins in a single sample. No specialized equipment is necessary.
- Product Insert (PDF, 453K)
- Troubleshooting Guide
- Assays for analytes represented in the Human Apoptosis Array Kit
- Western blotting antibodies for analytes related to the Human Apoptosis Array Kit
| Simultaneously detect the relative levels of these apoptosis-related proteins in a single sample. | ||
| Bad | TRAIL R1/DR4 | PON2 |
| Bax | TRAIL R2/DR5 | p21/CIP1/CDKN1A |
| Bcl-2 | FADD | p27/Kip1 |
| Bcl-x | Fas/TNFRSF6/CD95 | Phospho-p53 (S15) |
| Pro-Caspase-3 | HIF-1 alpha | Phospho-p53 (S46) |
| Cleaved Caspase-3 | HO-1/HMOX1/HSP32 | Phospho-p53 (S392) |
| Catalase | HO-2/HMOX2 | Phospho-Rad17 (S635) |
| cIAP-1 | HSP27 | SMAC/Diablo |
| cIAP-2 | HSP60 | Survivin |
| Claspin | HSP70 | TNF RI/TNFRSF1A |
| Clusterin | HTRA2/Omi | XIAP |
| Cytochrome c | Livin | |
General Assay Principle
Carefully selected capture antibodies have been spotted in duplicate on nitrocellulose membranes. Cellular extracts are diluted and incubated with the Human Apoptosis Array overnight. The array is washed to remove unbound proteins, followed by incubation with a cocktail of biotinylated detection antibodies. Streptavidin-HRP and chemiluminescent detection reagents are applied, and a signal is produced at each capture spot corresponding to the amount of protein bound. Chemiluminescence is detected in the same manner as a Western blot.
Kit Contents
- 4 Array Membranes
- 4-Well Multi-dish
- Array Buffers
- Lysis Buffer
- Wash Buffer
- Antibody Detection Cocktail
- Streptavidin-HRP
- Chemiluminescent Detection Reagents
- Transparency Overlay Template
- Detailed Protocol
For a complete list of the kit contents and necessary materials, please see the Materials Provided/Other Supplies Required sections of the product datasheet.